SiREM”s KB-1® Bioaugmentation culture used in “Microbial Cleavage of C–F Bonds in Two C6 Per- and Polyfluorinated Compounds via Reductive Defluorination” that was published in the Environmental Science & Technology first published online on Oct. 29, 2020.

Yujie Men (Department of Chemical and Environmental Engineering, University of California, Riverside) was the corresponding author, and her coauthors were Yaochun Yu, Kunyang Zhang, Zhong Li, Changxu Ren, Jin Chen, Yin-Hsuan Lin and Jinyong Liu.

Yujie Men is an Assistant Professor based in California whose research is focused on water quality systems engineering, environmental microbiology, microbial ecology and environmental chemistry.

Environmental Science & Technology (ES&T) is an impactful environmental science and technology research journal that aims to be transformational and direction-setting, publishing rigorous and robust papers for a multidisciplinary and diverse audience of scientists, policy makers and the broad environmental community. For more than 50 years, ES&T has been a foundational focus for thought-leading, policy-changing contributions and will continue to serve as the home for significant, broadly relevant, and generalizable research that serves to inform decision-making. The journal advances rigorous scholarship on complex environmental phenomena, particularly with respect to fate, transport, and transformation in natural and engineered systems, while simultaneously facilitating the solution of critical environmental problems. In addition to novelty and significance of research, ES&T considers the relevance of submitted manuscripts to its broad readership.ACS Publications” commitment to publishing high-quality research continues to attract impactful research from top authors around the globe. As the machinery of science continues to turn, ACS is committed to keeping pace. We continue to expand our offerings and improve our existing resources to help scientists accelerate their research and support their careers. A division of the American Chemical Society, ACS Publications supports researchers through journals, eBooks, scientific programs, and the news magazine Chemical & Engineering News.

Published Abstract

The C–F bond is one of the strongest single bonds in nature. Although microbial reductive dehalogenation is well known for the other organohalides, no microbial reductive defluorination has been documented for perfluorinated compounds except for a single, nonreproducible study on trifluoroacetate. Here, we report on C–F bond cleavage in two C6 per- and polyfluorinated compounds via reductive defluorination by an organohalide-respiring microbial community. The reductive defluorination was demonstrated by the release of F– and the formation of the corresponding product when lactate was the electron donor, and the fluorinated compound was the sole electron acceptor. The major dechlorinating species in the seed culture, Dehalococcoides, were not responsible for the defluorination as no growth of Dehalococcoides or active expression of Dehalococcoides-reductive dehalogenases was observed. It suggests that minor phylogenetic groups in the community might be responsible for the reductive defluorination. These findings expand our fundamental knowledge of microbial reductive dehalogenation and warrant further studies on the enrichment, identification, and isolation of responsible microorganisms and enzymes. Given the wide use and emerging concerns of fluorinated organics (e.g., per- and polyfluoroalkyl substances), particularly the perfluorinated ones, the discovery of microbial defluorination under common anaerobic conditions may provide valuable insights into the environmental fate and potential bioremediation strategies of these notorious contaminants.

More Information

Learn more about the article: https://pubs.acs.org/doi/10.1021/acs.est.0c04483
Learn more about ES&T: https://pubs.acs.org/journal/esthag
For consultation regarding SiREM”s Advanced Bioaugmentation Cultures, contact Jeff Roberts at jroberts@siremlab.com or visit: https://www.siremlab.com/advanced-bioaugmentation-cultures/