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SiREM’s Bioaugmentation Value Proposition: Guaranteed Results – Competitive Pricing – Unrivalled Technical Support

SiREM is a leader in the production, scale up and application of advanced bioaugmentation cultures to meet site remediation clean up levels.

Chlorinated Solvent Cultures

KB-1® and KB-1® Plus bioaugmentation cultures have been used for over a decade to enhance bioremediation of chlorinated solvents. These cultures introduce key microorganisms to contaminated sites where they are absent or in low concentrations. Bioaugmentation with KB-1® and KB-1® Plus is an effective solution to enhance remediation of a growing range of chlorinated solvents and other recalcitrant compounds including:

  • Chlorinated ethenes (PCE,TCE, all DCE isomers, VC)
  • Chlorinated ethanes (1,1,1-TCA, 1,2-DCA, TeCA and others)
  • Chlorinated methanes (CF, DCM)
  • Chlorinated propanes (1,2,3-TCP and 1,2-DCP)
  • RDX
  • Chlorofluorocarbons

Bioremediation at Lower pH

Many sites have an intrinsic pH below the optimum range for optimal bioremediation of chlorinated solvents (pH 6.5-7.8). For example, at pH’s below 6.0, slow and incomplete dechlorination of chlorinated ethenes is often observed, leading to accumulation of cDCE and VC. To meet our client’s needs, SiREM has developed a KB-1® Plus culture acclimated to dechlorinate ethenes at lower pH levels.

Consider-Low pH Acclimated KB-1® Plus;

  • Where aquifer pH is in the 5.8-6.3 range
  • In combination with aquifer neutralization to maximize dechlorination performance and/or reduce buffering needs
  • To maximize performance in poorly buffered aquifers where pH declines are likely due to dechlorination and fermentation of electron donors (both acid producing processes)

Benzene Culture

DGG-BTM is a specialized culture available for enhanced bioremediation of benzene under anaerobic conditions. Originally isolated from an oil refinery site, the culture couples benzene oxidation with methanogenesis. The culture converts benzene to methane and carbon dioxide, promoting degradation under methanogenic or sulfate reducing conditions. Methanogenic benzene degradation occurs syntrophically by benzene degraders, fermentative bacteria and methanogens. A Deltaproteobacterium, designated ORM2 has been identified as a key benzene degrading organism.

Other Cultures in Development

SiREM continues to actively perform research and development of novel bioaugmentation cultures for other emerging contaminants. Anaerobic degrading toluene, ethylbenzene, and xylenes cultures are currently being scaled up and are available for bench-scale treatability testing along with aerobic 1,4-dioxane degrading culture and cDCE/VC.

Anaerobic Toluene, Ethylbenzene, Xylenes Cultures

Groundwater contamination with Benzene, Toluene, Ethylbenzene and Xylenes (BTEX) is a globally significant, recalcitrant source of pollution. High costs and technical impracticability of conventional remediation strategies often results in BTEX contamination not being addressed where BTEX is found in deep groundwater and under anoxic conditions.

SiREM is continuing to develop additional anaerobic cultures for petroleum hydrocarbon sites. SiREM now has cultures for degrading toluene, ethylbenzene and xylene isomers which are scaled-up and available for treatability testing and pilot tests. These cultures – alone and in combination with our DGG-BTM anaerobic benzene culture are ideal for application to deep groundwater under anaerobic conditions.

Aerobic Degrading Cultures: 1,4-Dioxane and cDCE/VC

1,4 Dioxane has been widely used as a solvent and solvent stabilizer. It is frequently found at sites with chlorinated solvents such as 1,1,1-trichloroethane, 1,1-dichloroethene and trichloroethene, which can inhibit biodegradation making it challenging to remediate. SiREM’s 1,4-Dioxane degrading culture was isolated from a bioreactor, performs well in treatability studies degrading a range of 1,4-dioxane concentration (20 μg/L to 1,000 mg/L) to <3 μg/L. SiREM has culture available for treatability testing and field pilot tests.

cDCE and VC degradation are common groundwater contaminants and aerobic bioremediation options have been well documented in the literature. When found in highly aerobic aquifers and shallow groundwater, aerobic bioremediation approaches are often considered. SiREM has an aerobic cDCE and VC degrading culture in precommercial development available for bench scale treatability testing.

Contact SiREM to discuss aerobic bioremediation options available for your site.

Highest Quality Products

SiREM continues to actively perform research and development of novel bioaugmentation cultures for other emerging contaminants. Anaerobic degrading toluene, ethylbenzene, and xylenes cultures are currently being scaled up and are available for bench-scale treatability testing along with aerobic 1,4-dioxane degrading culture and cDCE/VC.

  • High concentrations of organisms
  • High contaminant mineralization rates
  • Culture stability
  • cVOC free
  • Pathogen/GMO free
  • Easy application using our customized delivery vessels, injection dispensers and tool kits

Bioaugmentation Saves Time & Money

Bioremediation is generally accepted as one of the least expensive remediation options (McDade et al., 2005) and bioaugmentation represents only a small fraction of project costs. Moreover, bioaugmentation is a one-time application that increases site efficiency by reducing cleanup time frames and associated site operation, maintenance and monitoring costs (Stroo et al., 2013). Bioaugmentation represents a modest investment that provides significant performance and financial dividends.

SiREM’s bioaugmentation cultures have been approved for use and applied in numerous jurisdictions including:

Worldwide Regulatory Acceptance

SiREM’s bioaugmentation cultures have been approved for use and applied in numerous jurisdictions including:

  • Forty-Five US States, Puerto Rico, Canada, seven EU countries, Israel, South Africa, Chile, Australia and Malaysia
  • KB-1® is the only culture of its type with regulatory approval for use in Canada
  • DGG-BTM has been field pilot tested in Canada and US
  • KB-1® and KB-1® Plus approved for use in Australia

Unrivalled Technical Support

As a recognized leader in applied bioremediation, SiREM leverages our bioaugmentation services with unrivalled technical support and related testing. The benefit is a seamless bioaugmentation experience with performance verification. Our technical experts are always available to help you achieve bioaugmentation success – contact us to get started with:

  • Pre-design consultation
  • Regulatory approvals
  • Field implementation
  • Data analysis and performance optimization

Experience the SiREM Difference

With SiREM you have an industry leader on your team that has your success as our first priority:

  • Site specific pre-bioaugmentation performance verification available
  • The only guaranteed bioaugmentation cultures in the industry
  • Fixed price quotations-includes shipping and incidentals
  • Bioaugmentation field technician support available

pdf For more information on KB-1®/KB-1® Plus
pdf For more information on KB-1® Plus for Remediation of Low pH Sites
pdf For more information on DGG-B Anaerobic Benzene Bioaugmentation Culture

pdf For more information on KB-1® Primer Preparation of anaerobic water for bioaugmentation culture injections