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Gene-Trac® Environmental Molecular Testing

Gene-Trac® testing is used to assess bioremediation potential and monitor enhanced bioremediation performance by quantifying and characterizing key dechlorinating bacteria in groundwater and soil/sediment from contaminated sites.

SIREM technician programing robot used to assemble Gene-Trac® reactions
Genetrac Dhc
Dhc concentration and spread after bioaugmentation at FE Warren AFB was monitored using Gene-Trac® Dhc (green contours) indicated varying concentration of Dhc over a large area of the Site (Figure courtesy of URS Corp.)

Use Gene-Trac® to:

  • Determine if suitable microorganisms are present for MNA remedies.
  • Predict the effectiveness of biostimulation before the addition of electron donor.
  • Determine the need for bioaugmentation.
  • Predict if intermediates such as cis-DCE or vinyl chloride are likely to accumulate.
  • Validate the performance of enhanced bioremediation projects.


Quantify and Characterize Environmental Microorganisms

Dehalococcoides (Dhc), Dehalobacter (Dhb) and Dehalogenimonas (Dhg) bacteria and key functional genes such as vinyl chloride reductase are critical to bioremediation of many chlorinated compounds, including chlorinated ethenes, ethanes, methanes and propanes. Gene-Trac® quantitative polymerase chain reaction (qPCR) testing is used to characterize and quantify these key dechlorinating bacteria at contaminated sites.

Gene-Trac® Tests Available

Test

Target

Relevance

Gene-Trac® Dhc** Dehalococcoides (Dhc) Critical to dechlorination of chlorinated ethenes such as PCE and TCE
Gene-Trac® VC* VC-reductase (vcrA) Key Dhc gene that dechlorinates vinyl chloride to ethene
Gene-Trac® Dhb Dehalobacter (Dhb) Critical in chlorinated methane (CF, DCM) and chlorinated ethane (e.g., 1,1,1-TCA) degradation
Gene-Trac® Dhg Dehalogenimonas (Dhg) Dechlorinate chlorinated propanes and 1,2-DCA
Gene-Trac® Universal All Bacteria For determining total bacterial biomass


Gene-Trac® Features

  • SiREM has license agreements for use of patented tests from Stanford University* and DuPont**
  • Comprehensive reports provide detailed testing and quality control parameters
  • Electronic data deliverables in various formats available
  • Field filter protocols are available that reduce shipping costs
  • Unparalleled technical support for data interpretation and planning

Contact SiREM to Learn More about how Gene-Trac® Testing Can Enhance Your Site Monitoring and Remediation Program

For more information on Gene-Trac® Testing