SiREM Gene-Trac® qPCR testing and Gene-Trac® RT-qPCR testing is accredited to ISO 17025:2017 through International Laboratory Accreditation Corporation (ILAC) and Perry Johnson Laboratory Accreditation (PJLA) for our Guelph, Ontario, and Knoxville, Tennessee, locations.

Click here to view Guelph and Knoxville ISO 17025:2017 Certificates

SIREM technician programing robot used to assemble Gene-Trac® qPCR reactions

The taxonomic composition of the microbial communities in the samples

Gene-Trac® testing is used to quantify key microorganisms and functional genes for the assessment of bioremediation potential and to monitor enhanced bioremediation performance. Several tests are available for quantifying and characterizing microorganisms and their respective functional genes in groundwater and soil/sediment from contaminated sites.

Use Gene-Trac® Testing to:

  • Determine if suitable microorganisms are present for MNA remedies.
  • Predict the effectiveness of biostimulation before the addition of electron donor.
  • Determine the need for bioaugmentation.
  • Determine the impact of site amendments including, electron donors/acceptors.
  • Predict if intermediates such as cDCE or vinyl chloride are likely to accumulate.
  • Characterize microbial community spatial and temporal variability.
  • Validate the performance of enhanced bioremediation projects.
  • Make informed decisions to manage bioremediation and other microbial systems.

Quantify Environmental Microorganisms and Microbial Communities

SiREM offers a growing number of targets for Gene-Trac® that uses quantitative polymerase chain reaction (qPCR) testing to quantify the key bacteria and functional genes that includes: Dehalococcoides (Dhc), Dehalobacter (Dhb) and Dehalogenimonas (Dhg), Desulfitobacterium (Dsb), Geobacter (Geo) and key functional genes such as vinyl chloride reductase (vcrA, bvcA), trichloroethene reductase (tceA), chloroform, 1,1,1-TCA and 1,1-DCA reductase (cfrA/dcrA). These microorganisms and functional genes are critical to reductive dechlorination of many chlorinated compounds, including chlorinated ethenes, ethanes, methanes, and propanes. Geobacter and sulfate reducers play key roles in the metabolism of aromatic hydrocarbon compounds and the generation of iron sulfur compounds critical to natural attenuation remedies. The dxmB and ALDH genes are important in the aerobic degradation of 1,4-dioxane. A full suite of tests are also available for both aerobic and anaerobic BTEX degrading organisms. Refer to the pdf document for the comprehensive list of Gene-Trac® tests that are available.

Gene Trac Tests

View a PDF of available Gene-Trac® Tests

Gene-Trac® Next Generation Sequencing (NGS)

Gene-Trac® NGS provides detailed microbial community composition to better understand the current and potential functions of microbial communities. Gene-Trac® NGS provides a non-targeted cost-effective insight into community function and dynamics to gain insight into complex microbial systems including bioremediation sites, wastewater treatment systems and landfills.

Use Gene-Trac® NGS to:

  • Understand dominant and potential microbial processes
  • Visualize microbial community spatial and temporal variability
  • Determine the impact of electron donors, nutrients, pH buffers, bioaugmentation, etc.
  • Identify inhibitory conditions and challenging site locations
  • Make informed decisions to manage bioremediation & other microbial systems

Gene-Trac® Features

  • Comprehensive reports provide detailed testing and quality control parameters
  • Electronic data deliverables in various formats available
  • USA or Canada sample reception options available
  • Field filter protocols are available that reduce shipping costs
  • Unparalleled technical support for data interpretation and planning

Use our online form or contact SiREM to place an order for sampling supplies or to learn more about how Gene-Trac® can enhance your site monitoring and remediation program

pdf For more information on Gene-Trac® Testing

pdf For more information on Gene-Trac® NGS